Objective To confirm the integration of transduced genes in the genomic DNA of host cells by Southern blot analysis. Methods The genomic DNA of transduced PA317/AIM and K562/AIM cells were extracted by phenol-chloroform method. The sequences of foreign genes were detected by polymerase chain reaction (PCR). The BamHI-digested DNA resolved by electrophoresis gel was transferred to nylon member and hybridized with the random primer labeled 32 P-DNA probe. The integration of transduced genes is thus d...