【摘要】 目的　研究胶质母细胞瘤中PTEN基因突变及增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA )的表达 ,并探讨其与两者之间的关系。方法　对 10 2例胶质瘤石蜡切片进行聚合酶链反应 单链构象多态性分析 (PCR SSCP) ,检测其PTEN基因突变情况 ;同时采用免疫组化方法检测PCNA在胶质瘤中的表达。结果　有 11例胶质母细胞瘤发生PTEN基因突变 ,突变发生率为 2 6 .2 % (11/42 ) ,显著高于其它胶质瘤 (χ2 =11.6 2 ,P <0 .0 1) ;胶质母细胞瘤PCNA阳性表达的平均指数为 45 .13 %±8.72 % ,其它胶质瘤的平均指数为 19.47%± 9.98% ,后者PCNA指数显著小于前者 (F =18.38,P <0 .0 1)。结论　PTEN基因突变在胶质母细胞瘤的发生、发展中起重要作用 ;脑胶质瘤中PCNA的表达程度与胶质瘤的恶性程度呈正相关 ;胶质母细胞瘤中PTEN基因突变与PCNA表达强度呈正相关更多还原

【Abstract】 Objective To research the PTEN gene mutation and PCNA expression in glioblastomas,and their relation.Methods The PTEN gene mutations were investigated by PCR SSCP(singl strand conformation polymorphism analysis of polymerase chain reaction products) in 102 archival,formalin fixed,paraffin embedded gliomas samples;also the PCNA expressions were investigated by immunohistochemistry.Results 11 of PTEN gene mutations were identified in glioblastomas,the ratio of PTEN gene mutation in glioblastomas is 26.2%(11/42),it is significantly different from that of the other gliomas(χ 2=11.62, P <0.01);the mean labeling index of PCNA positive expression in glioblastomas was 45.13%±8.72%,in other gliomas it was 19.47%±9.98%,the labeling index of PCNA positive expression in glioblastomas was significantly different from that in the other gliomas( F =18.38, P <0.01).ConclusionThe PTEN gene mutation may have an important effect on the generation and development of glioblastoma,the degree of PCNA positive expression in glioma has positive correlation with glioma’maligancy,and the PTEN gene mutation of glioblastoma has positive correlation with the degree of PCNA positive expression.更多还原