【摘要】 目的　探讨血清超氧化物歧化酶 (superoidedismutase ,SOD)和脂质过氧化产物丙二醛 (malonyldialdehyde,MDA)在围手术期心肌再灌注损伤中的浓度变化、相关性及临床意义。方法　心脏手术组 30例 ,分别在术前 1d ,主动脉开放后 10min ,术后 1d和 7d采血 ,化学比色法测定血清SOD和MDA。结果　心脏手术组SOD术前 (14 3 7± 2 2 ) μU/L ,再灌注 10min(113 5± 2 3 5 ) μU/L(P <0 0 1) ,术后 1d(14 1 3± 2 3 2 ) μU/L ,术后 7d(14 3 5± 2 4 2 ) μU/L ,MDA术前 (5 2 6± 2 19) μmol/L ,再灌注 10min(9 97± 3 6 8) μmol/L(P <0 0 1) ,术后 1d (7 17± 2 16 ) μmol/L ,术后 7d (5 75± 2 87) μmol/L ,并且血清SOD和MDA呈现负相关 (r =- 0 5 6 3,P <0 0 5 )。结论　CPB患者血清SOD活性下降 ,MDA水平增高 ,大量氧自由基的产生是造成心肌再灌注损伤的重要原因更多还原

【Abstract】 Objective To investigate clinical significance and the correlation between serum superoide dismutase(SOD) and malonyldialdehyde(MDA) during myocardial reperfusion injury under cardiopulmonary bypass(CPB). Methods SOD and MDA were detected quantitatively in CPB group( n =30). Blood samples were collected before operation(A),10 minutes(B), and day 1(C), day 7(D) after reperfusion. Results Concentrations of SOD were (143 7±22)μU/L, (113 5±23 5)μU/L,(141 3±23 2)μU/L and (143 5±24 2)μU/L at time A,B,C, and D, respectively. MDA values were (5 26±2 19)μmol/L,(9 79±3 68)μmol/L,(7 17±2 16)μmol/L and (5 75±2 87)μmol/L at time A,B,C and D,respectively. Concentrations of SOD decreased significantly at time B as compared with time A( P <0 01),while concentration of MDA increased significantly at the same time ( P <0 01). There was a negative correlation between the concentrations of SOD and MDA. Conclusion Serum SOD activity decreases and MDA value increases during CPB. Large amount of the production of oxide radicals is the important factor of myocardial reperfusion injury.更多还原