【摘要】 目的　研究糖皮质激素在妊娠后期肺表面活性物质合成过程中对糖皮质激素受体 (GR)的调节作用。方法　采用分子生物学杂交技术、Northern印迹法测定胎鼠肺及其二种主要细胞的GRmRNA及Wrang法测定外源性糖皮质激素对胎鼠肺及二种主要细胞GR结合活性的作用。结果　大剂量地塞米松可使胎鼠肺GR结合活性增加而GRmRNA无变化。在成纤维细胞 (FIB)培养液中加入 10 -7mol/L的可的松 ,GR结合活性和蛋白量增加 ,GRmRNA无变化。上皮细胞 (EPI)GR蛋白量减少 ,GRmRNA无变化 ,肺表面活性物质蛋白 (SP A)经同样处理其值增加。结论　胎鼠肺GR的调节发生于转译后水平 ,妊娠后期使用糖皮质激素对胎鼠的成熟有重要生理意义更多还原

【Abstract】 Objective To investigate the effect of glucocorticoid receptor(GR)in late gestation fetal lung around the time of the onset of augmented surfactant production. Methods GR mRNA was measured by Northern blots and hybridizations.GR binding activity was measured by Wrangs method. Results Maternal administration of a high dose dexamethatone increased fetal rat lung GR binding activity,without alteration in GR mRNA levels.Also incubation of fibroblasts (FIB)with 10 -7 mol/L cortisol increased GR immunoreactive protein and binding activity without affecting GR mRNA.Identical treatment, epithelial cells(EPI)were followed by decrease of GR protein without changes of GR mRNA. Conclusion The regulation of GR by glucocorticoid in fetal rat lung occurs at a posttranscriptional level,and increasing circulation glucocorticoid concentration during late gestation may be important to the maturation of fetal lung.更多还原