【摘要】 目的　寻找一种快速、准确、灵敏的人血清 β-人绒毛膜促性腺激素 (β- h CG)测定方法。方法　利用Access全自动微粒子化学发光免疫分析系统 (Access发光仪 )及其配套试剂测定β- h CG。结果　 Access发光仪常规测定 β- h CG线性范围 0～ 1 0 0 0 m IU/ml;批内 CV=1 .0 1 %,批间 CV=4.6 1 %;平均回收率1 0 0 .3 7%;与 RIA的比较试验 ,Y=1 .0 3 7XRIA- 3 .0 4 4 ,r=0 .992 9;88名非妊娠妇女参考值范围 0～ 2 .1 4m IU/ml。结论　 Access发光仪测定β- h CG快速、准确、灵敏 ,线性范围广 ,特别适合急诊检验更多还原

【Abstract】 Objective A rapid,accurate and highly sensitive method was established for determination of β human choriogonadotropin (β hCG) in serum.Method β hCG was measured by Access Immunoassay System.Results The linearity was 0～1000 mIU/ml.Within run CV and between run CV was 1.01% and 4.61% respectively.The average recovery ratio was 100.37%.A good correlation was shown as this method (Y) compared with RIA (X),Y =1.037 X RIA -3 044, r =0 992 9.The reference value was 0～2.14 mIU/ml in 88 cases non pregnant woman.Conclusion This assay was fast precise,sensitive,and had a wide linearity,especially suitable for an emergency measures.更多还原