节点文献

微量发色底物法测定血浆肝素浓度及临床应用

Determination and Clinical Application of Plasma Heparin Concentration by Micromethod Chromogenic Substrate

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 龚道元李子萍凌光鑫周克元易正山

【Author】 GONG Dao yuan 1,LI Zi ping 1,LING Guang xing 2,ZHOU Ke yuang 2,YI Zhen shan 2 (1.Department of Medical Laboratory,Fuoshan Professional Medical College of Guangdong Province Fuoshan 528000,China;2.Guangdong Medical College,Cuangdong Zhanjiang

【机构】 广东省佛山职工医学院检验系!广东佛山528000广东医学院检验系!广东湛江524000

【摘要】 目的 建立测定血浆肝素 (Hp)浓度方法 ,探讨其临床应用可行性。方法 用微量发色底物法测定血浆 Hp浓度 ,监测了 1 8例肾病病人血透期间和 2例 DIC病人在用肝素治疗期间血浆肝素浓度。结果 标准曲线在 0 .1~ 0 .8u/ml范围内成线性关系。高、中、低三种浓度回收率分别为 90 .1 %、93.2 %和91 .4%。批内 CV=5.2 % ,批间 CV=7.1 % ,与 TCT法有良好的相关性 (r=0 .91 89,P<0 .0 1 )。肾病病人血透期间血液 Hp浓度为 0 .2 7~ 0 .61 u/ml,2例 DIC病人在皮下注射 Hp后 2 h和 3h分别达到 0 .36u/ml和 0 .33u/ml的峰值浓度。结论 此法简便、快速、特异、稳定、准确性好 ,成本低 ,可用于临床。

【Abstract】 Objective To establish the method for assay plasma heparin(Hp) concentration and approach into the reliability of clinical application.Method Concentration of plasma Hp is determinated by micromethod chromogenic substrate and is oberserved in 18 hemodialysis patients suffering from in nephrosis and 2 DIC patients treated in Hp.Result The standard curve is linear in the 0 1~0 8u/ml range.The respective recovery rate are 90 1%,93 2%and 91 4% in hight,middle and low concentration.The within run CV is 5 2% and between run CV is 5 8%.There is a hight correlation with the result of the TCT( r =0 9189, P <0 01).Range of Hp concentration of hemodialysis patients is 0 27~0 61u/ml in a period of time of hemodialysis.The peak value of plasma Hp concentration of 2 DIC patients are 0 36u/ml and 0 33u/ml respectively for 2 and 3 hour after injecting Hp.Conclusion This method is simple,rapid,special,stable and accurate.The costs are reduced.It is suitable to clinical application.

  • 【文献出处】 陕西医学检验 ,Shaaxi Journal of Medical Laboratory Sciences , 编辑部邮箱 ,2001年01期
  • 【分类号】R446.1
  • 【下载频次】130
节点文献中: 

本文链接的文献网络图示:

本文的引文网络