【摘要】 应用检测淀粉酶分泌和单细胞[Ca2 + ]i 的技术 ,研究了Bt2-cGMP和GDP对柴胡皂甙(I)[SA(I)]和CCK -8促大鼠胰腺腺泡酶分泌和增加[Ca2 + ]i 的抑制作用。Bt2-cGMP对SA(I)和CCK -8促酶分泌的抑制有相似的剂量依赖性。Bt2 -cGMP对SA(I)刺激的酶分泌动力学的抑制较对CCK -8滞后并持续。SA(I)诱发的胰腺腺泡单细胞[Ca2 + ]i 的变化与CCK -8的作用有所不同 :[Ca2 + ]i 峰值上升较慢且持续较长 ,并在峰后[Ca2 + ]i 再次升高。GDP亦抑制SA(I)刺激的酶分泌和[Ca2 + ]i 增加的峰植。结果表明 ,SA(I)可激活胰腺腺泡细胞膜受体从而升高[Ca2 + ]i 和促酶分泌。更多还原

【Abstract】 The effects of Bt2-cGMP and GDP on Saikosaponin and CCK-8 stimulated amylase secretion and i increase were investigated in isolated rat pancreatic acinar cells. Bt2-cGMP inhibited both SA(I) and CCK-8-induced amylase secretions in a dose-dependent way. GDP also inhibited markedly saikosaponin-induced amylase secretion. SA(I) induced i increase in single pancreatic acinar cell was different from that induced by CCK-8: the rising phase of i increase was slow to develop, and lasted longer. In addition, there was a secondary increase after the initial peak. GDP significantly inhibited SA(I)-induces initial peak of i increase. These data indicate that SA(I) may activate i increase and amylase secretion via a direct action on the cell surface receptor.更多还原