【摘要】 针对人可溶性CD14 (sCD14 )的cDNA序列设计引物 ,通过反转录PCR技术 ,从U93 7细胞中 ,扩增出编码人可溶性CD14的基因序列 ,并将其克隆至质粒pGEM T中 ,通过PCR、酶切及序列测定鉴定重组载体。结果表明获得了人可溶性CD14基因片段 ,为下一步进行CD14表达及生物学活性研究奠定了基础。更多还原

【Abstract】 Human soluble CD14 (sCD14)cDNA fragment was amplified from total RNA extracted from U937 cells by using RT PCR method, the fragment was cloned into pGEM T Plasmid. Identified by PCR, endonuclease digestion and sequencing. Sequencing confirmed sCD14 gene fragment was obtained and laid the foundation for further research on expression and biological activities.更多还原