【摘要】 戊型肝炎病毒 (HEV)为单股正链RNA病毒 ,整个基因组有 3个开放性阅读框架 (ORF) ,ORF2 (全长约 1.98kb)是主要结构基因编码区。将经过PCR获得的ORF2基因插入非分泌型酵母穿梭质粒pPIC3,构成受醇氧化酶(AOX2 )的启动子与转录终止区控制的酵母表达质粒 ,重组质粒经酶切线性化后转化入酵母细胞GS115 ,经过鉴定可挑取与酵母染色体发生交换的重组体 ,用于进一步的真核表达更多还原

【Abstract】 Hepatitis E virus genome is a single?strained positive RNA which has three reading frames(ORF), ORF2 is the main structure gene code region. The ORF2 gene obtained from PCR can be cloned into intracellular expression vector pPIC3, then we will get a recombinant expression plasmid that controlled by promoter and transcription termination of AOX2. The recombinant plasmid integrated into GS115 by electroporation, and then analyze to confirm the integration of ORF2 gene.更多还原