【摘要】 端粒酶在四株人肝癌细胞中均有表达,而在正常人肝组织标本中却为阴性。在终浓度为1μmol/L时,针对端粒酶RNA组分的反义寡核苷酸对BEL-7404人肝癌细胞端粒酶活性具明显抑制作用,而正义和错义寡聚物则对该细胞端粒酶活性几乎没有影响。随着反义物浓度的降低,抑制作用亦随之减弱。用终浓度为5μmol/L的反义物处理培养的BEL-7404人肝癌细胞96h后,细胞形态发生变化。原位末端标记法(TUNEL)显示阳性标记细胞,流式细胞仪分析表明反义物作用后细胞凋亡率达到42.58%。更多还原

【Abstract】 Telomerase activity was detected in all of four human hepatoma cells but absent in normal liver tissue. Telomerase activity of BEL-7404 human hepatoma cells was inhibited effectively by antisense oligonucleotide to telom-erase RNA component at final concentration of 1μmol/L, whereas sense and missense oligonucleotides have no effects on its activity. The inhibition of telomerase activitywas weakened, as the concentration of antisense oligonucleotide decreased. Treating BEL-7404 human hepatoma cells 96 hours continuously by the antisense oligomers at final concentration of 5μmol/L, the niorphology of treated cells changed and apoptosis percent of the cells increased markedly.更多还原