【摘要】 利用反义核酸技术将端粒酶RNA的全长cDNA反向导入乳腺癌MCF 7细胞基因组中 .通过单细胞凝胶电泳实验发现 ,其DNA受H2 O2 损伤后的修复能力下降 .但端粒酶活性抑制为何引起其DNA损伤修复能力下降的原因尚待进一步研究 .更多还原

【Abstract】 By directing the synthesis of the telomeric repeats to the chromosome ends,telomerase prevented the decline of cellular proliferation with telomere abridging.Tumor cells,stem cells and some other cells showed high activities of telomerase.With antisense oligonucleotide technique,the whole cDNA of human telomerase RNA(hTR)was integrated inversely into human breast cancer cells MCF 7 in which antisense hTR RNA was expressed constantly.The results showed that the downregulation of telomerase activity significantly suppressed the DNA repair ability by single cell gel electrophoresis assay,though the mechanism was not clear yet.更多还原