【摘要】 为了检测细胞内源性Nmi和变异体Nmi s的表达、亚细胞分布以及可能形成的信号转导蛋白复合物 ,成功构建并表达了异源Nmi和Nmi s 获得纯化异源蛋白 ,用以制备兔源抗Nmi(Nmi s)多克隆抗体 .Western印迹在多种细胞株HL 60 ,K562 ,2 93T细胞中检测到Nmi(Nmi s)高表达 .在不同的细胞系中 ,Nmi表现为不同的蛋白复合物迁移带 ;Nmi(Nmi s)在胞浆和胞核均存在 ,而核内荧光更强 .Nmi(Nmi s)通过与多种蛋白相互作用行使其生物学功能 ,在不同细胞中可能与不同的蛋白相互作用 ,发挥不同的功能 .更多还原

【Abstract】 To detect endogenously expressed Nmi(Nmi\|s)and possible signaling complex in mammalian cell lines and to make certain their subcellular location,purified recombinant Nmi and its isoform were obtained in E.coli ,which were used to prepare polyclonal antibody.Nmi (Nmi\|s)were highly expressed in HL 60,K562 and 293T cell lines by Western blot.Nmi(Nmi\|s)could form large protein complexes exhibiting as different migrating band in separate cell lines.Nmi(Nmi\|s)distributed both in cytoplasm and nucleus,but more condensed in the nucleus.It could be concluded that Nmi(Nmi\|s)executed its function by associating with other proteins.It might interfere with different signaling pathways in various cell lines by forming certain protein complex.更多还原