【摘要】 将 h BDNF全长编码序列插入人 I型胶原基因 (Colia1)增强子 -启动子调节序列之下 ,构建了含 Colia1-BDNF微基因的重组体 p SCEPBFCAT,并转染人胚肌腱成纤维细胞。成功地利用了 Colia1基因调控元件介导 BD-NF基因在成纤维细胞中异位表达 ,SDS- PAGE显示表达产物分子量为 2 7k Da,免疫斑点杂交、Elisa和 Western杂交证实该表达产物具有 BDNF抗原活性更多还原

【Abstract】 The full length sequence encoding hBDNF was inserted down into the enhancer promotor regulatory element of Colia1 to construct minigene Colia1 BDNF. The recombinant eukaryotic expression plasmid pSCEPBFCAT was transfected into human fetal tendon fibroblasts in culture to realize ectopic expression of hBDNF gene in fibroblasts. The results of immuno dot blot, Elisa and Western hybridization confirm that the expression protein with apparent molecular weight 27 kDa in pSCEPBFCAT transfected fibroblasts possesses antigen activity specific for the antibodies to BDNF.更多还原