【摘要】 利用反相制备高效液相色谱结合溶剂萃取、大孔吸附树脂柱色谱和葡聚糖凝胶LH 2 0柱色谱方法 ,从管花肉苁蓉的乙醇提取物中纯化制备了苯乙醇苷类化合物松果菊苷的标准品 ,纯度达到 98%以上。方法操作简便 ,重现性好 ,可用于松果菊苷及其他苯乙醇苷类化合物的大量制备更多还原

【Abstract】 Echinacoside, one kind of phenylethanoid glycosides (PhGs), was isolated from the stems of Cistanche tubulosa (Schenk) R.Wight with a series of steps, including solvent extraction, D101 polymer adsorption column separation, Sephadex LH 20 separation, C 18 column reversed phase preparative high performance liquid chromatographic (RP prep HPLC) preparation and polyamide thin layer chromatographic detection The purity of the product was over 98% The chemical structure of echinacoside was identified by 1H NMR and 13 C NMR To find out the optimum condition of mobile phase of RP prep HPLC, several systems were used in this work Finally acetonitrile 1%HCOOH water (18∶82, V/V ) system was found to be the best On the other hand D101 polymer adsorption column and Sephadex LH 20 were also effective for PhGs separation更多还原