【摘要】 采用原位聚合的方法在毛细管中合成了非电荷型连续床电色谱原位柱 ,通过在电色谱流动相中加入阴离子表面活性剂十二烷基硫酸钠 (SDS)进行动态改性使其产生电渗流 ,考察了SDS浓度及有机改性剂浓度等因素对电渗流的影响。此类连续床柱制备容易 ,柱效可达 14万理论塔板 /m ,在不同的操作条件下有良好的稳定性 ,连续 10次运行 ,其死时间t0 与保留时间的精密度分别为 0 .2 2 %和 <0 .5 6 %。更多还原

【Abstract】 A monolithic capillary column was obtained by in situ co polymerization of butyl methacrylate with ethylene dimethacrylate. A mode of capillary electrochromatography (CEC) based on the dynamic adsorption of long chain sodium dodecyl sulfate(SDS)on the monolithic stationary phases was developed. Effects of SDS concentration and concentration of acetonitrile in mobile phase on the electroosmotic flow(EOF) of the column and the plate height on the electroosmotic velocity were investigated. Efficiency of monolithic capillary column with higher than 140?000 plates per meter for neutral aromatic compounds were obtained, and the relative standard deviations observed for dead time( t 0) and retention times of neutral solutes were about 0.22% and less than 0.56% for ten consecutive runs, respectively .更多还原