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从大蒜细胞中分离纯化出超氧化物歧化酶

Study on Extraction and Purification of SOD from Garlic Cells

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【摘要】 对从大蒜细胞中提取超氧化物歧化酶(Superoxidedismutase,SOD)的工艺过程进行了研究。通过对热变性法、等电点法、丙酮分级沉淀法和硫酸铵盐析法等几种方法的实验分析比较,找出一套操作简便SOD总收率高的分离纯化工艺。

【Abstract】 An extraction process to purify superoxide dismutase (SOD) from garlic cells was studied in this paper.By comparing the experimental results obtained by some different ways of treatment,such as heat treatment,isoelectric fractionation,acetone fractionation and (NH4)2SO4 salting out,a new and simple process was found to achieve the high SOD recovery with specific activity.

  • 【分类号】TQ914
  • 【被引频次】63
  • 【下载频次】762
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