【摘要】 荧光假单胞菌A4 6是通过紫外线诱变K10 0 5菌株所得到的一株抗噬菌体菌株。在噬菌体存在的情况下 ,使用菌株A4 6和K10 0 5在 50kL的发酵罐上进行了 2 酮基 D 葡萄糖酸的发酵生产。结果表明 ,荧光假单胞菌A4 6对噬菌体的抗性稳定 ,其发酵周期短 ,发酵转化率高 ;经过多次传代 ,荧光假单胞菌A4 6对噬菌体的抗性及其发酵生产 2 酮基 D 葡萄糖酸的能力没有改变 ;该菌株的发酵产物可以用于D 异抗坏血酸钠的合成中更多还原

【Abstract】 Pseudomonas fluorescens A46 was a phage resistant strain obtained from the mutation of the strain K1005 by using U.V. light as a mutagen. In the presence of phages, 2 keto D gluconic acid fermentation by Pseudomonas fluorescens A46 and its parent in 50?kL fermentor was studied. The results showed that Pseudomonas fluorescens A46 was indeed a phage resistant strain and the resistance was stable, with shorter fermentation period and higher fermentation conversion rate than its parent. After 17generation, its resistance against phages and 2 keto D gluconic acid producing ability had not changed. The fermentation product of Pseudomonas fluorescens A46 could be used in the synthesis of sodium D isoascorbate.更多还原