【摘要】 提出了一种地衣芽孢杆菌β 甘露聚糖酶制备的工艺。结果表明 :在设定的操作条件下 ,6 6L自控罐发酵酶活可达 2 6 0u/mL。发酵液用AlCl3与壳聚糖絮凝预处理后 ,絮凝体喷雾干燥可制成 90 0u/g的酶粉 ,适合饲料和造纸工业使用 ;絮凝上清液先以平均截留分子质量5万的中空纤维聚砜膜分离 ,透过液再用平均截留分子质量 1万的中空纤维聚砜膜浓缩后 ,喷雾干燥可制成 110 0 0u/g的酶粉 ,适合食品和医药工业使用。以上工艺酶活总收率 88% ,并易于工业放大更多还原

【Abstract】 A process has been developed for the preparation of β mannanase from Bacillus licheniformis  The enzyme activity was 260?u/mL when incubated in a 6 6L fermentor under the designed conditions After pretreatment of culture broth using flocculants of AlCl 3 and chitosan, the floc was spray dried (900?u/g) for use in feed or pulp industries, while the supernatant was fractionated by polysulfone membrane and then concentrated by polysulfone membrane The final product was spray dried (11?000?u/g) for use in food or pharmaceutical industries The proposed process resulted in the total yield of β mannanase activity of 88% and is easily scale up更多还原