【摘要】 植物遗传转化技术近年在农作物性状改良、植物生物反应器利用以及基因功能鉴定等方面得到了广泛的应用 .T -DNA转移是植物细胞农杆菌介导遗传转化整合和表达外源基因的基础 .农杆菌Ti质粒vir基因编码蛋白、农杆菌一些染色体基因编码蛋白及植物细胞一些基因编码蛋白或因子均参与T DNA转移 .转移过程包括农杆菌对植物细胞的识别、附着 ,细菌对植物信号物质的感受 ,细菌vir基因的诱导表达 ,T复合体的形成 ,跨膜运输 ,进核运输和整合等一序列过程 .植物细胞因子与农杆菌T DNA转移相关蛋白的相互作用最近被认为在T DNA转移过程中起重要作用更多还原

【Abstract】 The role of proteins encoded by related genes determined in the Ti plasmid virulence region ( vir genes), in the bacterial chromosome, as well as in the plant chromosome in the process of T DNA transfer were introduced. The process includes recognition and attachment, sensing of plant signals, activation of vir genes, generation of T DNA complex, T DNA complex export from the bacterial cell, import into the host plant cell nucleus and integration into the host genome.更多还原