【摘要】 实验通过大鼠侧脑室和杏仁核给予 5 HT3受体激动剂 1 phenylbiguanide (PBG) ,用 3H TdR掺入法测定脾细胞丝裂原 (concanavalinA ,ConA和lipopolysaccharide ,LPS)刺激增殖效应 ,用活化脾细胞增殖法测定IL 2生成 ,MTT法测定自然杀伤 (naturalkiller,NK)细胞活性和用放射免疫测定血浆皮质酮水平 ,以探讨大鼠杏仁核 5 HT3受体在免疫调控中的作用。结果表明 :5 HT3受体拮抗剂granisetron (GNT ,0 1～ 0 4mg/kgip)剂量依赖地增强ConA和LPS刺激的脾细胞增殖 ,作用在连续给药 5d最明显 ;双侧脑室给予PBG ( 5 μg/side)可增强ConA和LPS刺激的脾细胞增殖效应 ,作用在连续给药 3d最明显 ;双侧和单侧中央杏仁核给予PBG 0 5 μg均增强ConA刺激的脾细胞增殖和IL 2生成 ;底内侧杏仁核给予同剂量PBG仅增强LPS刺激的脾细胞增殖效应 ,不影响ConA刺激的脾细胞增殖和IL 2生成 ;中央杏仁核给予PBG升高血浆皮质酮的作用较底内侧杏仁核给予等量PBG引起的升高血浆皮质酮作用明显 (P <0 0 1)。侧脑室、中央杏仁核和底内杏仁核给予PBG对丝裂原刺激的脾细胞增殖效应影响不同 ,但均被同时同部位给予GNT所拮抗 ,提示杏仁核中央核和底内侧核的 5 HT3受体可能以不同方式参与ConA或LPS刺激的脾细胞增殖效应的调制更多还原

【Abstract】 The present study was to explore the possible immunomodulatory role of 5 HT 3 receptors in the amygdala in rats. Concanovalin A (Con A) and lipopolysaccharide (LPS) stimulated splenocyte proliferation response (SPR), production of IL 2, activity of natural killer (NK) cells and serum cortisol were measured by ~ 3H TdR incorporating method, MTT method and RIA, respectively. The Con A and LPS stimulated SPR was enhanced in a dose dependent manner by 5 HT 3 receptor antagonist granisetron (GNT) (0 1～0 4 mg/kg, ip). SPR was also enhanced by intracerebroventricular (icv) administration of 1 phenylbiguanide (PBG, 10 μg/d). Con A stimulated SPR and production of IL 2 were increased either by bilateral or by unilateral central amygdala (CeA) microinfusion of PBG (0 5 μg/side), but LPS induced SPR and NK cell activity were not affected. On the contrary, the LPS induced SPR was increased by either bilateral or unilateral basomedial amygdala (BmA) microinfusion of PBG (0 5 μg/side). The plasma cortisol level was significantly raised by CeA or BmA PBG microinfusion, but the effect induced by PBG intra CeA was greater than that induced by PBG intra BmA ( P <0 01). The effects of icv PBG and intra amygdala infusion were antagonized by granisetron. Asymmetrical modulation of immune reactivity by 5 HT 3 receptors in CeA or BmA was not observed in these experiments. It is suggested that 5 HT 3 receptors within the amygdala may modulate rat mitogen stimulated SPR in different manners.更多还原