【摘要】 目的探讨血浆中组织因子 (Tissue factor,TF)的检测对胃肠道肿瘤患者的临床意义。方法采用EL ISA法测定 32例胃癌、45例结直肠癌患者和 30例正常人血浆中 TF,并应用逆转录 -聚合酶链反应 (PT- PCR)检测这些患者和正常人外周血单核细胞中的 m RNA。结果两组消化道肿瘤患者血浆中的 TF均远远高于正常组 ,与病理分级并无相关性 ;采用 RT- PCR法在大部分肿瘤患者单核细胞中可检测到 TFm RNA,但在正常组中均不能检测到。结论肿瘤患者血浆中 TF升高可能是由于单核细胞合成增加所致 ,检测 TF在血浆中的水平和其m RNA的表达将有助于研究肿瘤发生发展的机制。更多还原

【Abstract】 Objective To investigate the serum level of tissue factor (TF) and mRNA expression in monocyte from patients with gastrointestinal tumor. Methods The TF level was determined in 32 gastric cancers, 45 colorectal carcinomas, 30 normal persons were determinated with ELISA, RT-PCR was used to detect expression of mRNA in monocyte. Results The high serum TF level was found in gastrointestinal tumor. TF mRNA in monocyte can be detected in most of cancer patients whereas it is undetectable in normals. Conclusions Expression of TF mRNA in monocyte probably accounts for high level of TF level in serum, TF value and mRNA expression can be a useful tool for early diagnosis and studying pathological processes.更多还原