【摘要】 目的　探讨基因重组转化生长因子α 绿脓杆菌外毒素融合蛋白 (TGFα PE40 ,简称TP40 )对人胰腺癌细胞生长的导向抑制作用。方法　用免疫组化LSAB法检测人胰腺癌SW 1990细胞表皮生长因子受体(EGFR)的表达 ,用结晶紫染色法和 3H 亮氨酸掺入法检测TP40对SW 1990细胞生长及蛋白质合成的抑制 ,用过量被生长因子 (EGF)竞争拮抗TP40的细胞毒作用。结果　SW 1990细胞免疫组化显示出大量棕黄色的EGFR阳性染色。TP40浓度为 1～ 10 0ng/ml时 ,对SW 1990细胞生长及蛋白质合成呈剂量依赖性抑制。过量EGF能完全拮抗TP40的细胞毒作用。结论　人胰腺癌SW 1990细胞能高水平地表达EGFR ,重组TP40对SW 1990细胞生长具有较强的抑制作用 ,作用部位为细胞的EGFR更多还原

【Abstract】 Objective To investigate the inhibitory effect of recombinant transforming growth factor α Pseudomonas exotoxin fusion protein (TGFα PE40;TP40) the growth of the on cultured human pancreatic carcinoma cells. Methods The Expression of epidermal growth factor receptor (EGFR) in the human pancreatic carcinoma SW 1990 cellswas analyseel with immunohistochemistry. The: methods Inhibitory effects of TP40 on growth and protein synthesi of the SW 1990 cells were analysed with crystal violet staining and 3H leucine incorporation. Excess EGF was addecl to bock the cytotoxic effects of TP40. Results The SW 1990 cells exhibited a large amount of brown immunoperoxidase reaction indicative of EGFR. When the concentration of TP40 was in the range of 1-100ng/ml,TP40 inhibited the growth of SW 1990 cells and protein synthesis in a dose dependent form. Excess EGF could completely block the cytotoxic of TP40. Conclusion The human pancreatic carcinoma SW 1990 cells can express EGFR at a high level. TP40 can significantly inhibit the growth of SW 1990 cells. The inhibitory effects of TP40 are specifically mediated by EGFR.更多还原