【摘要】 目的制备、研究抗乙肝iRNA前体脂质体。方法采用白细胞黏附抑制试验检测抗乙肝iRNA前体脂质体体外和小鼠口服后体内生物活性。结果抗乙肝iRNA前体脂质体口服有效 ,而iRNA和正常肝RNA前体脂质体均无作用。抗乙肝iRNA前体脂质体体外活性检测未黏附抑制指数在 5 3 .8%～ 76.2 % ;多批次供试品活性检测结果显示其稳定性和重复性较好。结论将抗乙肝iRNA制备为前体脂质体可成为有效的口服剂型更多还原

【Abstract】 PurposeSolid proliposome of anti hepatitis B immune RNA(iRNA) was prepared.MethodsBiological activity of anti hepatitis B iRNA wrapped in liposome against enzymolysis by RNase was determined by leukocyte adherence inhibition test in vivo in mice and in vitro. ResultsThe results indicated that anti hepatitis B iRNA wrapped in liposome, by oral administration could obviously inhibit the adherence of leukocyte in mice.Meanwhile,both of the nakded anti hepatitis B iRNA and the control RNA could not. In vitro ,the non adherence inhibition indexes of anti hepatitis B iRNA wrapped in liposome from various batches were 53.8%～76.2%.ConclusionThese results suggested that anti hepatitis B iRNA wrapped in liposome might be a promising oral preparation.更多还原