【摘要】 目的从烟曲霉菌培养上清液中分离纯化具有抗真菌活性的肽类物质 (FIP)。方法用离子交换柱层析法分离 ,用反相高效液相色谱法进一步纯化 ,经聚丙烯酰胺凝胶电泳分析确定相对分子质量。结果从烟曲霉菌培养上清液中分离出一种具有抗真菌活性的FIP。纯化后得到高活性的精制FIP ,其相对分子质量约为 8kD ,MTT法测定结果表明 ,FIP对 5种真菌均具有明显的抗真菌效应。结论烟曲霉菌培养上清液中含有抗真菌活性的肽类物质。更多还原

【Abstract】 PurposeThe aim is to purify the peptide with antifungal activity from Aspergillus fumigatus culture fluid.MethodsThe peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC,and the molecular weight was determined by tricine gel electrophoresis.ResultsA kind of peptide(FIP) with antifungal activity was separated. The molecular weight was about 8 000. The detection result by MTT method showed significant antifungal activity of the peptide to 5 kinds of fungi.Conclusion The study provided a reliable basis for developing drug and antiseptic.更多还原