【摘要】 目的对虎奶多糖进行硫酸酯化修饰 ,并研究其抗氧化活性。方法用氯磺酸 吡啶法对虎奶多糖进行硫酸酯化修饰得到硫酸酯化虎奶多糖 (S HNP) ;以Fe2 + Vc为氧自由基生成系统 ,体外观察S HNP对·OH引起的大鼠肝线粒体脂质过氧化损伤的保护作用 ;用化学发光法研究化学模拟体系CuSO4 Phen Vc H2 O2 DNA中S HNP对DNA损伤的保护作用 ;连苯三酚自氧化法观察S HNP对O·- 2 的清除作用。结果S HNP能显著抑制·OH引起的线粒体脂质过氧化产物TBARS的生成、线粒体的肿胀及膜流动性的降低 ;对·OH导致的DNA损伤有一定保护作用 ;对O·-2 有直接清除作用。结论S HNP具有较强的抗氧化活性。更多还原

【Abstract】 PurposeThe aim is to sulfonize Hunai polysaccharide from pleurotus tuber rigium (Fr.)Sing. and to evaluate the antioxidative activities of the sulfated polysaccharide (S HNP).Methods S HNP was prepared by the reaction of Hunai polysaccharide with chlorosulfonic acid Pyridine. The antioxidative activities of S HNP were evaluated as follows: (1) the inhibition effects on Fe 2+ Vc inducing the injury of rat liver mitochondria in vitro, (2) the protective effect on CuSO 4 Phen Vc H 2O 2 inducing the damage of DNA, (3) the scavenging effect on O ·- 2. ResultsS HNP could protect mitochondria from lipid peroxidation induced by Fe 2+ Vc, including the inhibitions of the increase of TBARS content, the swelling of mitochondria and the decrease of membrane fluidity, and protect DNA from the damage induced by CuSO 4 Phen Vc H 2O 2, and scavenge O ·- 2 generated in the self oxidation of pyrogallic acid. Conclusion S HNP exhibited marked antioxidative activities.更多还原