【摘要】 目的　探讨bcl 2反义寡核苷酸 (antisensephosphorothioateoligodeoxynucleotide,ASODN)对胃癌SGC 790 1细胞端粒酶活性的影响。方法　应用脂质体瞬时转染法介导bcl 2反义寡核苷酸 ,处理人胃癌细胞系SGC 790 1后 ,分别采用Northernblot、Westernblot检测bcl 2mRNA、蛋白表达 ,用端粒微孔板杂交法检测端粒酶活性。结果　bcl 2ASODN处理的胃癌SGC 790 1细胞端粒酶活性明显受到抑制 ,并与ASODN的浓度和处理时间有关。结论　bcl 2反义寡核苷酸可抑制胃癌细胞端粒酶活性更多还原

【Abstract】 Purpose To study the effects of bcl 2 antisense phosphorothioate oligodeoxynucleotide,(ASODN) on telomerase activity of gastric cancer SGC?7901 cell. Methods After gastric cancer SGC?7901 cell were treated by bcl 2 ASODN,we studies the expression of bcl 2 gene using Northern blot and Western blot,and telomerase activity using telomeric repeat amplification protocol(TRAP)?microtiter plate hybridization assay. Results bcl 2 ASODN can repress telomerase activity of gastric cancer SGC?7901 cell,and the effects of repression were related to treated time and dose. Conclusions bcl 2 ASODN can repress telomerase activity of gastric cancer cell.更多还原