【摘要】 目的　探讨小鼠L6 5 6 5克隆细胞株培养上清液中病毒的特性及其致病性。方法　从XC试验、逆转录酶活性、NB嗜性等方面了解培养上清液中病毒的特性 ;将培养上清液和超离心浓缩液分别皮下注射SSB系新生小鼠 ,观察其致白血病活性。结果　L6 5 6 5克隆细胞培养上清液的XC试验呈阳性 ,并具有逆转录酶活性和NB嗜性。培养上清液能诱发T淋巴细胞白血病 /淋巴瘤 (TL)和B淋巴细胞白血病 /淋巴瘤 (BL) ,发病率为 5 0 % (5只 / 10只 ) ;超离心浓缩液可诱发TL、BL和粒细胞白血病 (GL) ,发病率为 10 0 % (10只 / 10只 )。结论　小鼠L6 5 6 5白血病克隆细胞株培养上清液中存在具NB嗜性的、可诱发SSB系新生乳鼠TL、BL及GL的小鼠白血病病毒更多还原

【Abstract】 Purpose To study the virological and pathogenic characteristics in culture supernatant of murine L6565 clonal cells. Methods We studied the virological and biological characteristics of culture supernatant of murine L6565 clonal cells by XC assay,reverse transcriptase activity and NB tropic.The pathogenic characteristics of culture supernatant of murine L6565 clonal cells which was inoculated Subcutaneously into neonatal SSB strain mice. Results The culture supernatant of murine L6565 clonal cells showed positive with XC assay,reverstranscriptase activity and NB tropic test.This supernatant induced T lymphocytic leukemia/lymphoma(TL) and B lymphocytic leukemia/lymphoma(BL);the ultracentrifugal concentrated supernatant induced TL,BL and Granulocytic leukemia(GL). Conclusions There are murine leukemia viruses(MuLV) with NB tropic in the culture supernatant of murine L6565 clonal cells,which can induce TL and BL and GL on neonatal SSB strain mice.更多还原