【摘要】 为了研究上皮钙粘着蛋白 (E cadherin)对人肺腺癌细胞间粘聚和细胞悬浮生长的影响 ,利用基因重组技术构建了含全长上皮钙粘着蛋白cDNA的真核表达载体 ,通过脂质体法转染到A5 49肺腺癌细胞株中 ,用RT PCR和Western印迹鉴定并筛选上皮钙粘着蛋白高表达的细胞株 ,并观察转染前后肿瘤细胞间粘聚能力的改变以及细胞悬浮培养下的生长状态。结果表明转染细胞间聚集力比对照细胞明显增强 ,上皮钙粘着蛋白能够促进细胞悬浮生长的速度更多还原

【Abstract】 To explore the suppressive effects of Ecadherin on aggregation and anchorage independent growth of human lung adenocarcinoma cells, a mammalian expression vector containing full E cadherin cDNA was constructed and transfected into a metastatic human lung adenocarcinoma cell line A549. RT PCR and Western blot were used to analyse expression levels of E cadherin. Cells aggregation and anchorage independent growth of the tumor cells before and after gene transfection were assessed respectively. The results indicated that stable transfectants showed markedly increased levels of expressed E cadherin compared with the corresponding sham transfectants. The transfections showed more intense cellular aggregation and anchorage independent growth.更多还原