【摘要】 采用 2周龄强制断奶和 3周龄自然断奶的 2组裸鼠用皮下塞入法将手术切除的人肿瘤组织 (肿瘤表面组织和深部组织 )作异体移植 ,并将已长成的人肿瘤的异体瘤切除以残瘤的形式继续在裸鼠皮下增殖 ,观察移植瘤在不同周龄的裸鼠以及肿瘤组织取材差异的成瘤影响和残瘤的生长特性。结果表明 ,2周龄裸鼠移植瘤的成瘤性较 3周龄裸鼠为优 ,在相同移植天数 ,2周龄的成瘤期比 3周龄的裸鼠提前了约 4d,成瘤率也提高了一倍以上 ( P<0 .0 1 )。移植时取肿瘤表面组织比肿瘤深部组织的成瘤率提高 3倍以上 ( P<0 .0 0 5)。残瘤的传代率 1 0 0 % ,且成瘤期只有 8d。结果提示 ,使用 2周龄的裸小鼠可提高移植瘤的成瘤率 ,如接种时选择肿瘤表面组织更可提高移植瘤的成瘤率。在移植瘤成瘤后用残瘤的方式传代时 ,可使成瘤期提前更多还原

【Abstract】 The human gastric adenocarcinoma tissues with or without superficial tissue were subcutaneously transplanted into two weeks old ablactation nude mice or three weeks old weaning nude mice, then the formed heterogeneous tumor was excised and the remnant tumor continued to grow subcutaneously in nude mice, the differences of the tumor formation rate of the implanted tumor as well as the growing characteristics of remnant tumor in nude mice of different ages, and the effects of tissue selection of human tumor were observed. The tumor formation of implanted tumor is superior in two weeks old to three weeks old mice. In the same period, the tumor formation in 2 weeks old was 4 days before than in 3 weeks old mice, with two times higher in the tumor formation rate. Tumor formation rate in group of tumor tissue with capsule was three times higher than that without capsule. The passage rate of the remnant tumor was 100%, and the tumor forming time is only 8 days. It can be concluded that the tumor formation rate of implanted tumor can be improved by using two weeks old mice, it also can be much higher if using tumor tissues with superficial tissue. Tumor formation time can be shortened by using remnant tumor in passage after implanted tumor formed.更多还原