【摘要】 传统的DNaseⅠ足迹法程序繁杂 ,并且要求目的蛋白经过一定程度的纯化 .而固相DNaseⅠ足迹法通过结合有模板的磁珠 ,先富集序列特异的DNA结合蛋白 ,进行DNaseⅠ酶切 ,然后用测序胶分离并分析结果 .此方法简便易行 ,重复性好 ,并减少了对操作者的放射性损害 ,尤其适用于研究未经纯化的核蛋白粗提物内序列特异蛋白更多还原

【Abstract】 DNaseⅠ footprinting is labor intensive, time consuming and the target protein should be purified to some extent.Solid phase DNaseⅠ footprinting can enrich a sequence specific protein by target DNA immobilized onto paramagnetic beads. After DNaseⅠ digestion, separate the products by sequencing PAGE. This new method is quick ,simple and repeatable,which can minimize the exposure of the researcher to radiation. The solid phase approach may also facilitate the analysis of factors using crude protein mixtures.更多还原