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细菌人工染色体(BAC)及其分析和修饰方法简介
Advances in Analysis and Modification of Bacterial Artificial Chromosome
【摘要】 细菌人工染色体是一种新发展起来的DNA载体系统 ,它具有容量大、遗传特性稳定、易于操作等优点 .在基因文库构建和基因功能分析等方面有广泛的应用 .综述了近年来发展起来的对BAC进行分析和修饰的一些方法 .
【Abstract】 As a new developmental vector system, the bacterial artificial chromosome (BAC) have several advantages: larger capacity, hereditary stability and easy handling, etc. It has been extensively used in construction of genomic library and analysis of gene function. Some new methods of analysis and modification of BAC were reviewed.
【关键词】 细菌人工染色体;
定位修饰;
同源重组;
【Key words】 bacterial artificial chromosome; site directed modification; homologous recombination;
【Key words】 bacterial artificial chromosome; site directed modification; homologous recombination;
- 【文献出处】 生物化学与生物物理进展 ,Progress In Biochemistry and Biophysics , 编辑部邮箱 ,2001年03期
- 【分类号】Q71
- 【被引频次】22
- 【下载频次】490