【摘要】 以培养的人肝癌细胞系HepG2为研究对象 ,采用“冻干浓缩培养液载脂蛋白测定法” ,考察了地塞米松对HepG2细胞载脂蛋白 (apolipoprotein ,apo)AⅠ、AⅡ、CⅢ、B10 0及E分泌的影响 .结果表明 :地塞米松对HepG2细胞apoAⅠ和apoE的分泌有促进作用 ,对apoAⅡ、apoB10 0和apoCⅢ的分泌有抑制作用 ,且这种作用随地塞米松浓度的增加而增强 .当培养液中地塞米松的浓度为 5 5× 10 -5mol/L时 ,apoAⅠ和apoE的分泌分别增加 36 6 %和 49 4% (P <0 0 1) ,apoAⅡ、apoB10 0和apoCⅢ的分泌分别减少 38 9%、 31 9%和 2 9 8% (P <0 0 1) .更多还原

【Abstract】 In order to obersve the effect of dexamethasone on the secretion of apolipoproteins AⅠ,AⅡ,CⅢ,B100 and E by cultured HepG2 cells. The apolipoprotiens contents in culture media were measured by radioimmuodiffusion assay (RID) kits developed by authors research unit. 20 fold lyophilizely condensed culture media were used for the assays.The results showed that dexamethasone can increase the secretion of apoC and E, and inhibit the secretion of apoAⅡ,B100 and CⅢ;and the effect of dexamethasone were strengthened in a dose dependent manner. When the concentration of dexamethasone was 5 5×10 -5 mol/L in the culture media,the secretion of apolipoprotein AⅠand E increased 36 6% and 49 4%( P <0 01)respectively, while the secretion of apo AⅡ,B100 and CⅢ decreased 38 9%、31 9% and 29 8%( P <0 01)respectively.更多还原