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泰和乌骨鸡血清酯酶的多态性研究

Studies on the serum esterase polymorphism in Taihe chickens

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【作者】 贺淹才; 赵茹茜; 陈杰;

【Author】 HE Yan-cai, ZHAO Ru-qian,CHEN Jie (Key Lab of Animal Physiology and Biochemistry, Ministry of Agrieuhure, Nanhng Agricuhural Universitr,Nanjing210095,China)

【机构】 南京农业大学农业部动物生理生化实验室; 南京农业大学农业部动物生理生化实验室 南京210095; 南京210095; 南京210095;

【摘要】 应用垂直聚丙烯统胺凝胶电泳和数字成像扫描系统对42日龄的泰和鸡和AA(Arbor Aeres)肉鸡血清酯酶的多态性进行了分析。两种鸡的血清醋酶主要包括2个区域:Es- l和Es- 2。泰和鸡的Es- 1有4种类型:A型(A带出现,频率0.4575)、AB型(A带13带都出现.频率0.5415)、B型(B带出现,频率0.5365)和O型(A带B带均无,即无Es- 1,频率0.0732)。AA鸡的Es- 1有3种类型:A型(频率0.1667)、B型(频率0.5557)和O型(频率0.1667).两种鸡Es- 2区条带出现的频率均为1。AA鸡Es- 2的平均灰度(AA鸡vs泰和鸡201±15vsl74±14,p=0.029)总灰度(454079±669l0vs378968±66751,p=0.033)和净灰度(209517±4907vsl57873±24214,p=0.045)均显著高于泰和鸡,但Es- 1在品种间没有差异。两种鸡的Es- 2的平均灰度和净灰度都显著高于Es- 1(泰和鸡174±14vs150±10,p= 0.004;3432±29043vs157873±24214,p=0.001。AA鸡202±15vsl55±20,p=0.003;209517±4907vsll7776±58087,p=0.050),但Es- 2的总灰度值与Es- l的总灰度之间两种鸡均没有差异。

【Abstract】 Taihe ehicken is a Chinese indigenous breed with small body size, black skin and bone as well as silky white festher. The serum esterase isozyme polymorphism in 42-day-old Taiehe and Arbor Acres (AA) chickens were analyzed by verticsl polyacry-lamide gel electrophoresis and digital science image analysis soffware. Esterase phenotypes of the both chickens included 2 region; Es-l and ES-2. There were 4 types of Es-l in Taihe Chicken: type A (A band and, frepuency 0. 4878),type AB(A band and B band, frequency 0. 3415),type B(B band,frequency 0. 5365)and type O(no A band and B band, i. e. no Es-l,frequency 0. 0732) . There were 3 types of Es-l in AA broiler rtype A(frequency 0. 1667) ,type B(fre- puency 0. 5557)and type O (frequency 0. 1667). ES-2 band appear frequency were 1 in both varieties. ES-2 of AA broiler significandy higher than Taihe chick in scan mean imensi-ty of (201 ±15vs ±174 ±14, p = 0. 029), sum intensity (454079± 66910vs378968±66781,p = 0. 033 )and net intensitry(209817 ±4907vs 157873 ±24214 , p = 0. 045 ); but there were no differ- ence in Es-l in the two varieties, Mean intensity and net intensity of ES-2 were significantly higher than that of Es-l in both varieties (Taihe 174±14vsl50±10, p = 0. 004; 3432 ±29043vsl57873 ±24214 ,p = 0. 001. AA broiler 201 ±15vs155 ± 20,p ; 0. 003 ;209817±4907vsl17776±58087 ,p = 0. 050) ;but there was no difference between sum intensitr of ES-2 and Es-l in both varieties.

【关键词】 泰和鸡; 酯酶; 多态性; 表现型;
【Key words】 Chinese Taihe chicken; esterase; phenotype; polymorphism;
【基金】 国家自然科学基金资助项目(39670545)
  • 【文献出处】 上海交通大学学报(农业科学版) ,Journal of Shanghai Jiaotong University (Agricultural Science) , 编辑部邮箱 ,2001年03期
  • 【分类号】S865.34
  • 【被引频次】1
  • 【下载频次】32
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