【摘要】 报道了一种改进的毛细管电泳柱后化学发光检测装置 .将现行装置中的光电倍增管光电流检测器改成噪声和暗电流更小、灵敏度更高的光电倍增管光子计数器 ,反应毛细管末端出口处设置一引流装置 ,解决了由于柱后液滴形成而引起基线规律性波动的问题 .利用改进后的装置 ,对铁 ( )的检出限可达 1 .0× 1 0 - 10 mol/L(2 .0× 1 0 - 18mol)比灵敏度最高的 FIA-ICP- MS低 1 0倍 .方法快速简便 ,适合于痕量物质的检测 .更多还原

【Abstract】 A newly improved apparatus of end-column chemiluminescence detection system for capillary electrophoresis has been described. In the present method, the photon counting method was used as the signal recovery instead of the originally current detection method and a drainage fluid unit was put behind the reaction capillary. Comparatively sharp and symmetric peak of Fe(Ⅲ) ion,the sensitive catalyst for the chemiluminescence reaction of luminol and H 2O 2,are obtained with the detection limit of 1.0×10 -10 mol/L.更多还原