【摘要】 为建立一种可分泌人胶质细胞源性神经营养因子 (GDNF)的工程细胞 ,研究其在帕金森病基因治疗中的可能作用。克隆携带 Kozak序列的人 GDNFc DNA,并转染至人类神经母细胞瘤细胞系 SH- SY5 Y细胞 ,将此工程细胞与大鼠原代多巴胺能神经元共培养 ,免疫组化检测多巴胺能神经元。发现工程细胞可防止多巴胺能神经元退变死亡 ,有助于多巴胺能神经元抵抗 MPP+ 的毒性损伤。表明可分泌人 GDNF的工程细胞在帕金森病基因治疗中可能具有重要的应用价值更多还原

【Abstract】 To construct a kind of engineered cells that can secrete human glial cell line-derived neurotrophic factor(GDNF)and study its possible effects on gene therapy of Parkinson’s diseaseCloned the GDNFcDNA with Kozak sequence by RT-PCR,and then transfected it into SH-SY5Y cell line,co-cultured the engineered cells with primary mesencephalic cellsResults showed that the engineered cells showed obvious protecive effects such as anti-degeneration and anti-MPP+lesionGDNF produced by the engineered cells was of full biological functionIt suggests that the engineered SH-SY5Y cells can secrete human GDNF and could play an important role in gene therapy of Parkinson’s disease更多还原