【摘要】 从病变鸡法氏囊组织中分离纯化IBDV ,提取其基因组RNA .以IBDVRNA为模板 ,进行反转录反应合成cDNA第 1链 .采用PCR技术扩增VP5基因 .将PCR产物经酶切后与 pcDNA3.1 (+)载体连接 ,经转化、筛选得到重组质粒 pIBDV VP5.对VP5基因进行了测序并对其序列进行了分析 .更多还原

【Abstract】 Chicken infectious bursal disease viruses (IBDV) were isolated and purified from infected bursa and then the viruses were used to extract genomic RNA.The cDNA first strand was synthesized by reverse transcription using IBDV genomic RNA as the template.A DNA fragment coding for the viral protein 5(VP5) of IBDV was amplified by PCR,which was ligated with pcDNA3.1(+) vector following restriction enzyme digestion.The ligates were transformed into XL1 blue and the recombinant plasmid pIBDV VP5 was screened and identified.The sequencing for VP5 gene and its sequence analysis were performed.更多还原