【摘要】 以甜瓜基因组ＤＮＡ为模板，利用ＰＣＲ技术扩增获得甜瓜ＡＣＣ氧化酶基因家族成员ＣＭ－ＡＣＯ１的前导顺式元件，长度为 ８８６ｂｐ。将其克隆至ｐＧＥＭ－Ｔ ｅａｓｙ载体上。序列分析结果表明：克隆的 ＣＭ－ＡＣＯ１基因顺式元件的核苷酸序列与已报道的相比有９９．６％同源，元件中含ＢＲＥ，ＥＲＥ，ＷＵＮ等，能接受多种外界信号刺激。更多还原

【Abstract】 An 866 bp fragment of the 5’ non-translated region of ACC oxidase gene CM-ACO1 was amplified by PCR using total genomic DNA from melon as template. The amplified fragment was cloned into the plasmid of pGEM T easy and sequenced. The resultant nucleotide sequence had a homology of 99 % as compared with the reported sequence. Motifs of BRE, ERE, WUN with significant homology to known cis-acting elements were identified in the cloned nucleotide sequence. Analysis revealed that these elements were in response to various stress.更多还原