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日本血吸虫雌雄成虫可溶性蛋白组分的双向电泳分析

Soluble Proteins analysis of male and female Schistosoma japonicum by two-dimensional gelelectrophoresis

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【作者】 吴忠道冯明钊徐劲孟玮阮志燕余新炳

【Author】 Wu Zhongdao 1, Fung Mingchiu 2, Xu Jin 1, Mong Wei 1, Ruan Zhiyian 1, Yu Xinbing 1 1 Sun Yat-sen University of Medical Sciences,Guangzhou 510089 2 Department of Biology, the Chinese University of Hong Kong

【机构】 中山大学中山医学院寄生虫学教研室香港中文大学生物系中山大学中山医学院寄生虫学教研室 广州510089广州510089广州510089

【摘要】 目的 分析日本血吸虫雌雄成虫可溶性蛋白组分。方法 双向电泳法。结果 用双向电泳分析雌雄虫体多肽斑点分别有 310个和 2 90个 ,匹配的蛋白点为 5 1个。日本血吸虫雌雄成虫可溶性蛋白中存在一些共同多肽斑点 ,但不同的多肽斑点较多。结论 日本血吸虫雌雄成虫可溶性蛋白组分存在较大差异 ,值得进一步研究。

【Abstract】 Complementation of male and female worms is essential for the maturation of adult worms in Schistosoma japonicum . Although several sex specific genes of S.japonicum were identified, the major difference between the male and female worm in protein level have not been studied. In this study, we reported the analysis of the soluble proteins of the male and female worms by two-dimensional gelelectrophoresis. The 2D-gel electrophoresis revealed 310 and 290 polypeptides in female and male respectively. 51 polypeptides were the same as identified by the 2-D analysis programme. This unexpectedly great difference between the male and female worms in protein level requires further studies.

【基金】 国家自然科学基金 (No:30 0 0 70 6 83) ;国家教育部博士点基金 (No :2 0 0 0 45 );广东省自然科学团队研究项目资助
  • 【文献出处】 热带医学杂志 ,Journal of Tropical Medicine , 编辑部邮箱 ,2001年02期
  • 【分类号】R383.24
  • 【被引频次】16
  • 【下载频次】91
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