【摘要】 目的 探讨血液透析患者丙型肝炎病毒（ＨＣＶ）的检测方法。方法 对７９例尿毒症长期血液透析患者，采用荧光定量ＰＣＲ法测定血清ＨＣＶ－ＲＮＡ水平，及第二代酶免疫试验（ＥＩＡ）检测丙型肝炎病毒抗体（抗－ＨＣＶ）。结果 血液透析患者抗－ＨＣＶ阳性率２２．８％（１８／７９），荧光定量ＰＣＲ法ＨＣＶ－ＲＮＡ检出率３９．２％（３１／７９）。在抗ＨＣＶ阴性的患者中，ＨＣＶ－ＲＮＡ的检出率为４２．６％（２６／６１）。两种方法检测结果的符合率为５０．６％（４０／７９）。结论 荧光定量 ＰＣＲ技术可弥补ＥＩＡ检测的不足，在抗－ＨＣＶ阴性的血液透析患者中检测 ＨＣＶ ＲＮＡ具有重要意义。更多还原

【Abstract】 Objective The aim of the paper is to study the detection of HCV in patients of haemodialysis. Methods Serum from 79 haemodialysis patients were tested for hepatitis C viral RNA titers by amplisensor quantitative PCR and Anti-HCV by secondary enzyme immune assay (EIA-2). Results Positive rate of anti-HCV in haemodialysis patients was 22. 8%. Positive rate of HCV - RNA by amplisensor quantitative PCR was 39. 2%. Of anti-HCV negative patients, positive rate of HCV RNA by amplisensor quantitative PCR was 42. 6% . The coincidence rate of the two methods is 50. 6% Conclusions Amplisensor quantitative PCR could make up weakness of EIA . It is necessary to detect HCV RNA in haemodialysis patients of anti-HCV negative.更多还原