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碱蓬下胚轴试管再生植株的研究
STUDIES ON HYPOCOTYL CULTURE IN VITRO AND PLANTLET REGENERATION OF SUAEDA GLAUCA
【摘要】 碱蓬 [Suaedaglanca (Bunge)Bunge]幼苗的下胚轴接种在MS加不同浓度NAA、2 ,4-D、6 -BA和IAA的培养基上 ,愈伤组织诱导频率为 94.6 4%~ 10 0 % .愈伤组织转移到以 6 -BA为主的分化培养基上 ,不定芽分化频率为 2 5 .2 5 %~ 6 0 % .不定芽转入IBA生根培养基后 ,10d左右生根 ,得到再生植株 .
【Abstract】 Hypocotyl of common seepweed [Suaeda glauca (Bunge) Bunge] seedling were cultured on four MS agar media of different concentrations NAA、2, 4-D、6-BA and IAA. The calli were induced on the media. The induction frequency of calli amounted to 94.64%~100.00%.The calli were transferred to medium that contained higher concentration 6-BA. The induction frequency of adventitious buds were from 25.25% to 60%. Adventitious buds were transferred to the MS medium that contained higher concentrations IBA. After ten days, the calli grew roots and got plantlet regeneration in vitro.
- 【文献出处】 曲阜师范大学学报(自然科学版) ,Journal of Qufu Normal University(Natural Science) , 编辑部邮箱 ,2001年01期
- 【分类号】Q943.1
- 【被引频次】4
- 【下载频次】70