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碱蓬下胚轴试管再生植株的研究

STUDIES ON HYPOCOTYL CULTURE IN VITRO AND PLANTLET REGENERATION OF SUAEDA GLAUCA

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【作者】 王兴安马宗琪侯元同张举仁张慧山赵彦修

【Author】 WANG Xing_an ①, MA Zong_qi ①, HOU Yuan_tong ①, ZHANG Ju_ren ②, ZHANG Hui ③, ZHAO Yan_xiu ③ (①Department of Biology, Qufu Normal University, 273165, Qufu; ②Department of Biology,Shangdong University,250100, Jinan; ③Institute Plan

【机构】 曲阜师范大学生物系!273165曲阜市山东大学生物系!250100济南市东师范大学逆境植物实验所!250014山东省济南市山东师范大学逆境植物实验所!250014

【摘要】 碱蓬 [Suaedaglanca (Bunge)Bunge]幼苗的下胚轴接种在MS加不同浓度NAA、2 ,4-D、6 -BA和IAA的培养基上 ,愈伤组织诱导频率为 94.6 4%~ 10 0 % .愈伤组织转移到以 6 -BA为主的分化培养基上 ,不定芽分化频率为 2 5 .2 5 %~ 6 0 % .不定芽转入IBA生根培养基后 ,10d左右生根 ,得到再生植株 .

【Abstract】 Hypocotyl of common seepweed [Suaeda glauca (Bunge) Bunge] seedling were cultured on four MS agar media of different concentrations NAA、2, 4-D、6-BA and IAA. The calli were induced on the media. The induction frequency of calli amounted to 94.64%~100.00%.The calli were transferred to medium that contained higher concentration 6-BA. The induction frequency of adventitious buds were from 25.25% to 60%. Adventitious buds were transferred to the MS medium that contained higher concentrations IBA. After ten days, the calli grew roots and got plantlet regeneration in vitro.

【关键词】 碱蓬下胚轴试管培养再生植株
【Key words】 common seepweedhypocotylin vitroplantlet regeneration
  • 【文献出处】 曲阜师范大学学报(自然科学版) ,Journal of Qufu Normal University(Natural Science) , 编辑部邮箱 ,2001年01期
  • 【分类号】Q943.1
  • 【被引频次】4
  • 【下载频次】70
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