【摘要】 报道采用咖啡因和热休克相结合的方法 ,抑制受精卵第二极体释放诱导太平洋牡蛎三倍体的优化方案。实验结果表明 ,三倍体诱导率随着咖啡因浓度的增高而增高 ,D形幼虫的孵化率却随着咖啡因浓度的增高而降低 ;所进行 30～ 36℃热休克温度试验 ,其对三倍体诱导率影响不大 ,但对孵化率影响明显。在 2 3～ 2 5℃条件下 ,当 50 %受精卵出现第一极体时 ,用浓度为 2 g/ L的咖啡因 ,结合 33℃± 1℃的热休克 ,处理牡蛎受精卵 15min,三倍体诱导率达 80 %以上 ,孵化率在 4 5%左右。通过此法诱导的三倍体群幼虫的成活与生长情况与其二倍体群体无明显差异。更多还原

【Abstract】 Triploidy was induced in Pacific oyster, Crassostrea gigas using caffeine combined with thermal shock and the optimal treatment was investigated. The triploid rate increased while the hatching rate of D-shaped larvae decreased with the increase of the concentration of caffeine used. The temperature of thermal shock had less effect on triploid rate but more on hatching rate. The optimal concentration of caffeine and temperature of thermal shock were 2g/L and (33±1)℃ respectively. Treating eggs for 15min under the optimal treatment when 50% eggs showed that polar body I at 23~25℃ could yield over 80% of triploid rate and about 45% of hatching rate of D-shaped larvae. There was no significant difference in survival and growth between triploid larvae and its control group.更多还原