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栽培番茄耐盐变异系的离体选择

In Vitro Isolation of Salt-Tolerant Variant of Cultivated Tomato (Lycopersicum esculentum Mill)

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【作者】 杨永杰董树刚付成秋刘志鸿吴以平

【Author】 Yang Yongjie 1 Dong Shugang 1 Fu Chengqiu 1 Liu Zhihong 2 Wu Yiping 1 (1 College of Marine Life Science,Ocean University of Qingdao, Qingdao, 266003) (2 Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Science Qingdao,

【机构】 青岛海洋大学海洋生命学院!青岛266003中国水产科学研究院黄海水产研究所!青岛266071

【摘要】 利用细胞工程技术 ,首先在 1.5% Na Cl下进行筛选 ,然后在 1.0 % Na Cl下长期继代及在无盐下和 1.5% Na Cl下反复的多步选择方法。经过 7个月的连续筛选 ,分离得到耐盐性稳定的番茄耐盐变异细胞系。耐盐系在不同 Na Cl浓度下的生长情况表明 ,该耐盐系的耐盐性有了较大提高 ,接近于 1.0 % Na Cl。耐盐系和对照中可溶性糖和游离脯氨酸含量有明显差异 ,这与耐盐系具有较高耐盐性相对应。同时对游离脯氨酸在盐胁迫下积累的意义进行了探讨

【Abstract】 From the cell line derived from the cotyledon and hypocotyl of seedlings of tomato( Lycopersicum esculentum Mill), a stable NaCl tolerant variant was successfully isolated by the combination use of 1.5% NaCl one step and 1.0% NaCl multi step screening last for 7 months.The FW increment of variant and wild cell in different NaCl concentrations indicated that the salt tolerance of variant is approaching 1.0% NaCl, while the wild cell died almost completely. The physiological analysis of variant and wildcell showed notable differences. Under the stress of different NaCl concentrations, the former’s soluble sugar and free proline content were much higher than the latter’s, this helped the variant adjusted itself to the NaCl stress better. However, the inconsistency of the FW increment and the accumulation of free proline content under higher NaCl concentrations, showed that the active function of the accumulation of free proline under salt stress is limited in a certain NaCl concentrations. When overstress happened, it might only act as a physiological index of salt injury.

【关键词】 番茄耐盐性变异系筛选生理性指示
【Key words】 tomatosalt tolerancevariantscreeningphysiological inH
【基金】 山东省科委项目!(鲁科字 93- 2 4)资助
  • 【文献出处】 青岛海洋大学学报(自然科学版) ,Journal of Ocean University of Qingdao , 编辑部邮箱 ,2001年01期
  • 【分类号】S641
  • 【被引频次】32
  • 【下载频次】198
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