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大豆球蛋白基因转化桑树获得转基因植株
Transgenic Plants via Transformation of Glycinin Gene to Mulberry
【摘要】 利用农杆菌介导法将大豆球蛋白AlaBlb亚基基因导入桑树组织,经抗性筛选和组织培养获得再生植株。通过对转基因植株的PCR-Southern杂交及RNA点杂交等鉴定,证实大豆球蛋白基因转化桑树成功。
【Abstract】 The transgenic plans transformed with glycinin gene AlaBlb using Agrobacterium fumefaciens mediation method were regenerated from mulberry tissue through resistance selection and tissue culture. The results from PCR-Southern and RNA hybridization confirmed that glycinin gene AlaBlb was introduced into mulberry.
【关键词】 大豆球蛋白AlaBlb亚基基因;
桑树;
遗传转化;
【Key words】 glycinin gene AlaBlb; mulberry; genetic transformation;
【Key words】 glycinin gene AlaBlb; mulberry; genetic transformation;
【基金】 国家自然科学基金资助项目(39570556)
- 【文献出处】 农业生物技术学报 ,Journal of Agricultural Biotechnology , 编辑部邮箱 ,2001年04期
- 【分类号】S888.31
- 【被引频次】15
- 【下载频次】104