【摘要】 从怀孕 １７ ｄ母兔取得 ３个胎儿，分离培养得到 ３个胎儿成纤维细胞系 ＲＦＦ１、ＲＦＦ２和 ＲＦＦ３。通过扩增兔 ｓｒｙ基因核心序列片段对３个细胞系的性别进行鉴定（Sｒｙ－ＰＣＲ），结果表明：ＲＦＦ１和ＲＦＦ３为雄性，ＲＦＦ２为雌性。进一步利用染色体核型分析法鉴定３个细胞系的性别，与Ｓｒｙ－ＰＣＲ法的结果完全一致。因此表明本实验所用的Ｓｒｙ－ＰＣＲ的引物、细胞基因组ＤＮＡ的提取方法、ＰＣＲ反应条件是合适的，可以快速准确地鉴定体外培养的兔胎儿成纤维细胞的性别。更多还原

【Abstract】 Three 17-day old rabbit fetus were collected and their fibroblasts were isolated, then cultured and passaged into three cell lines : RFF l, RFF2, RFF3. The sex of these three cell lines were determined by amplifying core sequence of rabbit sry gene. The result indicated that RFF l and RFF3 are male, while RFF2 is female. Further determination by Chromosome karyotyping supported the result of Sry-PCR. So it demonstrated that the method of Sry-PCR used in this experiment can rapidlly and precisely determine the sex of cultured rabbit fibroblast cell lines.更多还原