【摘要】 以近等基因系Ｈ７Ｒ和Ｈ７Ｓ为材料，运用差别显示（ＤＤ－ＰＣＲ）技术获得ＲＭａ１９片段。用此片段为探针筛选ｃ ＤＮＡ文库，获得 ３ ９４４ ｂｐ全长基因 ＲＩＭ２。Ｎｏｒｔｈｅｒｎ杂交结果显示该基因在水稻抗感品系中均受稻瘟病菌的强烈诱导。ＲＩＭ２ ＯＲＦ编码 ６５３个氨基酸，其序列与多种植物转座类似蛋白有 ３２％～５５％的同源性。其 ５＇端约 ８００ ｂｐ与 Ｘａ２１基因家族Ａ１，Ｃ的非编码区有８２％的序列同源性。Ｓｏｕｔｈｅｒｎ杂交表明ＲＩＭ２多拷贝存在与水稻基因组中。ＲＩＭ２为一新的受病菌诱导的早期反应基因。更多还原

【Abstract】 A rice gene RIM2 was identified by mRNA differential display, it was strongly induced by both incompatible and com- patible strains of Magnaporthe grisea. A 3.9 kb cDNA clone was identified and predicted to encode a 653 amino-acid protein, which is 32%~55% identical with proteins encoded by the CACTA transposons of other plants.AT-rich region was found in the 5’ and 3’ noncoding termini of RIM. Sequence of RIM2 was 82% identical with sequences flanking the Xa21 gene family members, Al and C, the disease resistance genes. Southern blot hybridization with genomic DNA from different rice isolines indicated that RIM2 is present in multiple copies. These results suggest that RIM2 might be involved in the early rice response to infection induced by the fungal pathogen.更多还原