【摘要】 枯草芽孢杆菌 （Ｂａｃｉｌｌｕｓ　ｓｕｂｔｉｌｉｓ） ＳＯ１１３对水稻白叶枯病菌 （Ｘａｎｔｈｏｍｏｎａｓ　ｏｒｙｚａｅ　ｐｖ．ｏｒｙｚａｅ）有强烈的抑菌作用（表现出一透明抑菌圈）。于培养液上清中加入６０％饱和度的硫酸铵沉淀所得的蛋白粗提液对热稳定，对链霉蛋白酶Ｅ不敏感，对蛋白酶 Ｋ部分敏感，对中国各稻区由叶枯病菌的７种致病型都有强烈的抑杀作用。蛋白粗提液１００℃加热 １５ ｍｉｎ后脱盐，经ＤＥＡＥ－Ｓｅｐｈａｒｏｓｅ Ｆａｓｔ Ｆｌｏｗ柱层析得 ３个未分开的抗菌活性峰，未吸附部分经 ＣＭ－Ｓｅｐｈａｒｏｓｅ Ｆａｓｔ Ｆｌｏｗ柱层析，得到 １个主活性峰。由于Ｂ．ｓｕｂｔｉｌｉｓ ＳＯ１１３的分泌蛋白对水稻白叶枯病菌表现出良好的广谱抗性以及丰富的抗菌活性峰，因此对其进一步的研究和设法克隆编码抗菌蛋白的基因都具有重要意义。更多还原

【Abstract】 In in vitro tests, Bacillus subtilis SO113 showed strongly amibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo) (showing a clear inhibition zone, anti-Xoo). A crude antibacterial preparation (CAP) was obtained by adding ammonium sulphate to supernatant fluid of B. subtilis SO113 to a final saturation of 60% and displayed strong killing activity to 7 pathotypes of Xoo which covers all rice cropping-region in China. The anti-Xoo activity of CAP is thermostable, resistant to proteinase E, and partially sensitive to proteinase K. One and three peaks with anti-Xoo activity were obtained from CAP, which previously heated at 100℃ for 15 min and salts was removed from, after DEAE-Sepharose Fast Flow and CM-Sepharose Fast Flow, respectively. It is significant to clone the genes encoding those anti-Xoo peptides because the anti-he peptides from B. subtilis SO113 showed extensively inhibitory activities against 7 pathotypes of he in China.更多还原