【摘要】 高等植物细胞内含有肌动蛋白 ,但含量较低 ,难于提取及进行进一步研究。我们用 PCR的方法扩增肌动蛋白 c DNA,并克隆到表达质粒 p Trp His,然后转化大肠杆菌 DH5α;在 IPTG诱导下 ,肌动蛋白表达在包涵体中 ,在没有 IPTG诱导时 ,肌动蛋白表达在胞液中更多还原

【Abstract】 In higher plant, the concentration of actin is very low, therefore the purification and study of actin are rather difficult. We amplified actin gene cDNA clone PEAcⅠ coding region by the method of PCR and constructed expression plasmid with plasmid pTrpHis A. Actin gene expressed in the inclusion bodies under the induce of IPTG, whereas in the cytosol under non IPTG induced condition.更多还原