【摘要】 目的 :研究端粒酶在切除食管癌标本中的活性。方法 :采用端粒酶重复扩增 (TRAP) -微孔板杂交法 ,对 15 0例切除食管癌标本癌中央组织、癌旁 1cm、3cm、以及 5～ 6 cm处 4块组织进行端粒酶活性分析。结果 :端粒酶在食管癌组织中央部位的阳性检出率为 98% ,癌旁 1cm处组织中的阳性率为 72 % ,癌旁 3cm处的阳性率为 42 % ,癌旁 5～ 6 cm处的正常组织阳性率为 4%。结论 :端粒酶活性在切除食管癌标本中的差异性分布对判断食管癌切除范围以及预后等具有一定的指导意义。更多还原

【Abstract】 Objective:To study the activity of telomere enzyme in esophageal carcinoma.Method:Activity analysis of telomere enzyme on sections of esophageal carcinoma in 150 cases,with 4 sections,one being from the center;one,1cm by the carcinoma;one,3cm and one,5~6cm using the method of TRAP-random Millipore mating.Result:Positive detectable rate of telomere enzyme in the center part was 98.5%;1cm by it,72%; 3cm,42%;the positive detectable rate in the normal tissue 5~6cm away from carcinoma was 4%.Conclusion:The determination of the telomere enzyme activity in esophageal carcinoma sections can be expected to be the judge for resection scope and the prognosis criterion.更多还原