【摘要】 用电脉冲法处理小鼠精子后进行体外受精 ,体外受精卵的卵裂率和桑椹胚发育率都有所降低 .以 1 .0 kv/ cm,3 0 μs的最佳处理条件处理过的处理组其卵裂率和桑椹胚发育率分别为 63 .5%和 50 .9% ,与对照组的 84 .1 %和 80 .0 %相比 ,有显著差异 (p<0 .0 1 ) ,说明电脉冲处理对精子受精率和胚胎的体外早期发育有影响 .将 1 .0 kv/ cm,3 0μs和 4 .8kv/ cm,90μs电脉冲处理后的精子与正常对照组 (离心后重悬于电脉冲缓冲液 )的精子分别离心固定后制作电镜切片 .观察结果发现 ,电脉冲处理组的顶体外细胞膜发生了膨胀 ,覆盖顶体的细胞膜陷入 ,在顶体后区有细胞膜的断裂现象 .其变化率分别是 :1 .0 kv/ cm,3 0 μs处理组为 3 6.3 % ;4 .8kv/ cm,90 μs处理组为 4 6.7% .较低电压脉冲处理组精子顶体部分发生了类似顶体反应的变化 ;而高压电脉冲处理组的线粒体发生了空泡化和线粒体的大小不均一性变化更多还原

【Abstract】 The percentage of both IVF of mouse oocytes and the development of mouse IVF embryo were lowered as sperm was treated with electroporation. The rate of cleavage and morula were 63.5% and 50.9% in treatment group, and 84.1% and 80.0% in control group respectively. There was a significant difference between two groups. The results indicated that electroporation was relative to the rate of IVF and the development of early stage IVF embryos. The sperms treated with electroporation and in control were made into electron micrograph sections. The results showed that plasma membrane of mouse sperm treated with electroporation swelled and sank inside, and some broken plasma membrane were found at rear part of acrosome. The rates of change were 36.3% in 1.0kv/cm/30μs and 46.7% in 4.8kv/cm/90μs respectively. The sperms treated with low voltage appeared a similar acrosome reaction. The mitochondria of sperms treated with electroporation appeared with cavity, and non-homogenous of mictochondria observed in high voltage treatment.更多还原