【摘要】 将克隆的番茄 ACC合成酶基因的反义 RNA-核酶嵌合的 DNA序列用 Hind 和 Kpn I切割后重组于植物表达载体 p GA643中 ,用三亲融合法导入农杆菌 LBA4 40 4中 ,采用叶盘法转化番茄子叶 ,诱导出再生小植株 ,获得了卡那霉素的抗性植株 .提取植物总 DNA,用p GA643作探针 ,通过斑点杂交 ,已筛选出整合有外源基因的工程植株 .为进一步研究该嵌合基因对 ACC合成酶基因表达的影响及获得商品化的转基因番茄奠定了基础 .更多还原

【Abstract】 The cloned antisense RNA Ribozyme chimeric DNA sequence against tomato ACC synthase gene was inserted into a binary vector pGA643 digested with KpnI and HindⅢ,to construct an expressing vector.Transgenic tomato plants were obtained by Agrobacterium mediated transformation of cotyledons of tomato,and confirmed by dot blotting analysis,using pGA643 as probe.更多还原